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| Title |
Bacteriophage-mediated Biocontrol of Escherichia coli O157:H7 in Soil
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| Type of Resource |
still image
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| Date Created |
2008-04-23
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| Digital Origin |
born digital
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| Rights Statement |
http://digital.uwyo.edu/copyright.htm
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| Keyword (topic) |
TBD
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| Series Title |
Undergrauate Research Day 2008
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| Creator(s) |
Bolenbaugh, Kyle E.
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| Genre |
Powerpoint/Pdf
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| Publisher |
University of Wyoming
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| Place of publication |
Laramie, Wyoming
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| Language |
eng
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| Summary |
Escherichia coli O157:H7 continues to be a major foodborne pathogen. Recent outbreaks have occurred in ready to eat (RTE) fruits and vegetables that have been attributed to the presence of E. coli O157:H7 in soil. These recent outbreaks have perpetuated a need for increased control measures against pathogenic bacteria in soil. Bacteriophages, viruses which only infect bacteria, provide a safe, natural option of biocontrol against these pathogens. A cocktail of six bacteriophages specific against E. coli O157:H7 was developed and tested as a practical means for reducing this bacterium in soil. Broth tests using a multiplicity of infection (MOI) of 100 showed the complete elimination of an E. coli O157:H7 culture with a 105 CFU/ml concentration within an hour with no evidence of resistance. Soil samples were inoculated with 106 CFU/mL in water and kept moist until cell counts stabilized. The bacteriophage cocktail was utilized to treat the soil at a MOI of 25. No significant reduction in E. coli O157:H7 was observed in the soil. The complex makeup of the soil likely retards the spread of bacteriophage and renders the control measure less effective. However, the efficacy in broth shows the great potential of bacteriophage as a biocontrol and certainly supports the need for further exploration.
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| Notes |
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
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