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| Document Title |
Differentiation between infections caused by Brucella abortus and Yersinia enterocolitica 0:9 in Elk by Western Blot.
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| Type of Resource |
still image
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| Date Created |
2009-05-18
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| Digital Origin |
born digtal
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| Rights Statement |
http://digital.uwyo.edu/copyright.htm
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| Keyword (topic) |
elk brucellosis
elk yersiniosis
cross-reactivity
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| Series Title |
Undergrauate Research Day 2009
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| Creator(s) |
Thompson, Samantha
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| Contributor(s) |
Andrews, Dr Gerard
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| Publisher |
University of Wyoming
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| Place of publication |
Laramie, Wyoming
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| Language |
eng
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| Summary |
Brucellosis is a disease which impacts both domestic and wildlife animal populations. Elk have been misdiagnosed with brucellosis by the standard serologic laboratory assay (ELISA), because of potential cross-reactivity with non-Brucella antigens. This phenomenon is likely due to similarity of the composition of the O-polysaccharide side-chain of lipopolysaccharide (LPS) with Yersinia enterocolitica. Chronic infection by this enteric species in elk may, therefore, confound assay results. To address this problem, we examined LPS from Y. enterocolitica and B. abortus by immunoblot against sera from elk, infected with Y. enterocolitica O:9. LPS probed with sera from Yersinia-infected animals showed equally strong reactivity to o-polysaccharide from both species. We next evaluated the anti-Yersinia and Brucella serum samples against a purified recombinant Yersinia protein, LcrV, with no known homolog in B. abortus. Six of 8 samples from Yersiniainfected elk were positive, while none of the Brucella-POS samples showed specificity for LcrV. Conversely, all 8 anti-Yersinia samples were negative against a protein unique to Brucella, AfuA, while 8 of 9 anti-Brucella samples were positive. We conclude that that cross-reactivity can be abrogated through the use of protein antigens unique to each species. A modified/augmented assay may therefore delineate between yersiniosis, brucellosis, or co-infection.
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| Notes |
From - Undergraduate Research Day 2009 - Celebration of Research - Abstracts
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