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| Title |
Antibiotic Production in Wyoming Soil Actinomycetes
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| Type of Resource |
still image
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| Date Created |
2008-04-23
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| Digital Origin |
born digital
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| Rights Statement |
http://digital.uwyo.edu/copyright.htm
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| Keyword (topic) |
TBD
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| Series Title |
Undergrauate Research Day 2008
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| Creator(s) |
Botchway, Lady C.
Jennings, Holly
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| Genre |
Powerpoint/Pdf
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| Publisher |
University of Wyoming
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| Place of publication |
Laramie, Wyoming
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| Language |
eng
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| Summary |
The purpose of the experiment was to identify actinomycetes collected from soil and to determine their effectiveness in producing antibiotic compounds against Escherichia coli, Staphylococcus epidermidis, and Bacillus subtilis. Actinomycetes in soil samples from Bud Love Wildlife Reserve outside of Buffalo, Wyoming were isolated and cultured on one side of half strength Standard Methods agar plates and allowed to grow at 37º C for seven days. E. coli, S. epidermidis, and B. subtilis were streaked perpendicular to the actinomycetes cultures and allowed to grow at 37º C for seven days. The plates were analyzed by measuring zones of inhibition due to antibiotic activity. Morphology of unknown Streptomyces samples producing antibiotics was examined. DNA of the samples was isolated, cloned by PCR, and compared.
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| Notes |
From - Undergraduate Research Day 2008 - Celebration of Research - Abstracts
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